Antiviral Res 2011 Dec;92 (3): 493-6. [IF:4.439]
Protective immunity elicited by a pseudotyped baculovirus-mediated bivalent H5N1 influenza vaccine.
Wu Q , Xiao S , Fan H , Li Y , Xu J , Li Z , Lu W , Su X , Zou W , Jin M , Chen H , Fang L .
Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
华中农业大学农业微生物学国家重点实验室
Abstract
The development of novel H5N1 influenza vaccines to elicit a broad immune response is a priority in veterinary and human public health. In this report, a baculovirus vector was used to construct bivalent recombinant baculovirus vaccine encoding H5N1 influenza virus hemagglutinin proteins (BV-HAs) from clade 2.3.4 and clade 9 influenza viruses. Mice immunized with 5×10(7)IFU BV-HAs developed significantly high levels of H5-specific neutralizing antibodies and cellular immunity that conferred 100% protection against infection with H5N1 influenza viruses. This study suggests that baculovirus-delivered multi-hemagglutinin proteins might serve as a candidate vaccine for the prevention of pre-pandemic and pandemic H5N1 influenza viruses.
